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84968611
10X TAE (Tris Acetate EDTA) - Sterile, 0.22um Filtered - 500 mL
99% of respondents would recommend this to a friend
Nº de artículo:
84968611
CRC 22880
Price Details
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What Stands Out
Sterile Solution
Ensures contamination-free experiments, providing reliable results in molecular biology applications.
0.22um Filtered
Removes unwanted particles effectively, maintaining high purity levels for sensitive analyses.
Generous Volume
500 mL capacity offers extensive use for repeated experiments, reducing the need for frequent replenishment.
Detalles de producto
| UPC | 791689523521 |
| Manufacturer | UFC Bio |
| Container Type | Bottle |
| Item Form | Liquid |
| Brand Name | UFC Bio |
| Number of Items | 1 |
| Included Components | Sterile 10X TAE Buffer |
| Manufacturer Part Number | BTAE-10X |
| Unit Count | 16 Fluid Ounces |
| Item Weight | 1.2 lbs (540 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biology experiments, providing reliable buffer conditions for DNA and RNA electrophoresis.
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Laboratory Technicians
Perfect for technicians needing a sterile, ready-to-use buffer for consistent results across multiple experiments.
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Educational Institutions
Comprehensive solution for educational labs generating clear results in studies involving gel electrophoresis.
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General Use
Not suitable for applications outside molecular biology, where other buffers may be more effective or affordable.
DESCRIPCIÓN DEL PRODUCTO
10X TAE (Tris Acetate EDTA) - Sterile, 0.22um Filtered - 500 mL
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Preguntas y respuestas de los clientes
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Pregunta:
What is 10X TAE and what is its primary use?
Respuesta: 10X TAE (Tris Acetate EDTA) is a buffer solution widely used in molecular biology. It primarily serves to maintain a stable pH and ionic strength during techniques such as electrophoresis of nucleic acids. Its formulation allows researchers to separate DNA and RNA fragments effectively, ensuring integrity and visibility of the samples during analyses. -
Pregunta:
How is 10X TAE prepared for use in experiments?
Respuesta: To prepare 10X TAE for practical use, dilute it to a 1X solution with distilled water before use. This dilution allows for optimal buffering capacity during procedures like gel electrophoresis. It’s essential to mix thoroughly to ensure uniform concentration, which significantly enhances the performance of the electrophoresis process. -
Pregunta:
Why is the 0.22um filtering important for 10X TAE?
Respuesta: The 0.22um filtration process is crucial as it removes potential contaminants such as bacteria and particulates that could interfere with molecular biology experiments. Ensuring sterility helps protect sensitive reactions and enhances the integrity of experimental results, especially in applications like cloning and sequencing. -
Pregunta:
Can 10X TAE be stored, and if so, how?
Respuesta: Yes, 10X TAE can be stored for extended periods. To maintain its integrity, keep it in a cool, dark place, ideally refrigerated. Make sure the cap is tightly sealed to prevent any contamination or evaporation. This storage method preserves its buffering capacity and ensures reliable performance in future experiments. -
Pregunta:
Can I use 10X TAE for both DNA and RNA electrophoresis?
Respuesta: Absolutely, 10X TAE is suitable for electrophoresis of both DNA and RNA. Its buffering properties help maintain the integrity of nucleic acids during separation. This versatility makes it a preferred choice in many laboratories for various applications such as PCR, cloning, and RNA analysis. -
Pregunta:
What are the safety considerations when using 10X TAE?
Respuesta: When handling 10X TAE, it's essential to use personal protective equipment such as gloves and goggles to avoid skin and eye contact. Although this buffer is non-toxic, adhering to standard laboratory safety protocols ensures a safe working environment during experiments. -
Pregunta:
Is 10X TAE compatible with all types of gel electrophoresis?
Respuesta: Yes, 10X TAE is compatible with a variety of gel types, including agarose and polyacrylamide gels. Its ionic strength and pH stability enhance resolution during the separation of nucleic acids, making it suitable for various applications in biological research and education. -
Pregunta:
What is the difference between 10X TAE and 1X TAE?
Respuesta: 10X TAE is a concentrated buffer solution that must be diluted to a 1X concentration for practical use in experiments. The primary difference lies in their usage, with 10X being utilized for storage and preparation, while 1X is employed in actual laboratory procedures, ensuring optimal conditions for nucleic acid analysis. -
Pregunta:
How does the pH of 10X TAE affect my experiments?
Respuesta: The pH of 10X TAE is typically around 8.0, which is essential for maintaining the stability of DNA and RNA during electrophoresis. A stable pH ensures that nucleic acids remain negatively charged, facilitating their movement through the gel matrix. Fluctuations in pH can lead to poor separation outcomes or degradation of samples. -
Pregunta:
Where can I buy 10X TAE (Tris Acetate EDTA) - Sterile, 0.22um Filtered - 500 mL in Costa Rica?
Respuesta: You can purchase 10X TAE (Tris Acetate EDTA) - Sterile, 0.22um Filtered - 500 mL from Ubuy in Costa Rica. Ubuy offers an extensive selection of laboratory supplies and reagents, ensuring you get quality products that meet your scientific needs.
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CRC 22880
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