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73677904
Tissue RNA Miniprep Kit with DNase I, 50 preps
86% of respondents would recommend this to a friend
Nº de artículo:
73677904
CRC 167119
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The EasyPrepTM Tissue RNA Kit provides an easy and fast method for isolating total RNA from tissues and cultured cells within 30 minutes, purifying up to 100 µg of total RNA ready for various applications.
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What Stands Out
Quick RNA Isolation
Streamlined protocol allows rapid extraction of high-quality RNA from tissue samples, minimizing processing time and enhancing lab productivity for researchers and clinical labs.
DNase I Included
Comes with DNase I for effective removal of genomic DNA contamination, ensuring pure RNA for accurate downstream applications such as qPCR and sequencing.
High Yield
Designed to deliver optimal RNA yields from even challenging tissue types, providing reliable results for critical experiments and enhancing reproducibility across studies.
Detalles de producto
Product Technical Details
| Package Dimensions | 10 x 10 x 6 inches |
| Item Weight | 3 pounds |
| Manufacturer | Bioland |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists working with RNA extraction from various tissue types for downstream applications.
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Researchers in Genomics
Recommended for genomics researchers who need high-quality RNA for sequencing and expression studies.
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Academic Institutions
Great for academic labs requiring a reliable method for RNA isolation for teaching and research purposes.
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Budget-Conscious Users
Not suitable for users with limited budgets, as this kit may be more expensive compared to competitors.
DESCRIPCIÓN DEL PRODUCTO
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Preguntas y respuestas de los clientes
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Pregunta:
What is the Tissue RNA Miniprep Kit with DNase I used for?
Respuesta: The Tissue RNA Miniprep Kit with DNase I is designed to extract high-quality RNA from various tissue samples. This kit streamlines the purification process, ensuring that you obtain pure, intact RNA suitable for downstream applications such as RT-PCR, qPCR, and sequencing. It includes DNase I to eliminate any contaminating DNA that could interfere with your RNA analysis. Researchers often use this kit in molecular biology studies to investigate gene expression or perform RNA profiling from animal tissues. -
Pregunta:
How does the DNase I in the kit benefit RNA purification?
Respuesta: The inclusion of DNase I in the Tissue RNA Miniprep Kit is crucial for breaking down any residual genomic DNA, which can skew results in RNA-based experiments. By preventing DNA contamination, the RNA obtained can provide more accurate insights into gene expression levels. This is especially important in studies where distinguishing between RNA and DNA is vital, such as in quantitative PCR assays or when assessing RNA integrity for sequencing applications. -
Pregunta:
Can this kit be used for different types of tissues?
Respuesta: Yes, the Tissue RNA Miniprep Kit with DNase I is versatile and can be used on various tissue types, including animal tissues like liver, heart, and brain, as well as plant tissues. Its protocol is optimized to ensure efficient RNA recovery from both types, which is essential for researchers working across different biological fields. Whether you’re analyzing gene expression in mammalian cells or extracting RNA from plant samples, this kit provides reliable results. -
Pregunta:
Is prior experience in molecular biology necessary to use this kit?
Respuesta: While some familiarity with molecular biology techniques can be advantageous, the Tissue RNA Miniprep Kit is designed to be user-friendly. Step-by-step protocols and clear instructions allow both novice and experienced researchers to successfully extract RNA. Users can easily follow the detailed guidelines to ensure optimal results, making it suitable for a wide audience. Many educational institutions and laboratories utilize this kit for teaching and research purposes alike. -
Pregunta:
What volume of tissue sample is required for the extraction process?
Respuesta: The Tissue RNA Miniprep Kit typically requires around 30 mg to 100 mg of tissue sample depending on the type and quality of the tissue. This amount ensures that there is sufficient material to extract a viable RNA yield for analysis. It's important to weigh the tissue accurately before proceeding with extraction, as both under-sampling and over-sampling can affect the quality and yield of the RNA, which is vital for downstream applications. -
Pregunta:
What type of RNA is extracted using this kit?
Respuesta: The Tissue RNA Miniprep Kit is designed to extract total RNA, which includes messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). Extracting total RNA is crucial for a comprehensive understanding of gene expression as it provides all RNA species involved in the protein synthesis process. Researchers aiming to profile gene expression or perform RNA-seq will find this kit particularly valuable due to its ability to retain all types of RNA in the sample. -
Pregunta:
Can this kit be used for formalin-fixed, paraffin-embedded (FFPE) samples?
Respuesta: The Tissue RNA Miniprep Kit can be optimized for use with FFPE samples, but specific adjustments in the protocol may be necessary. FFPE tissues often require additional steps for de-paraffinization and may have degraded RNA due to fixation. For optimal results, following adapted protocols tailored for processed samples is essential. This kit provides flexibility, and many researchers successfully isolate RNA from FFPE tissues for retrospective studies in cancer research and pathology. -
Pregunta:
How long does the RNA extraction process take using this kit?
Respuesta: The RNA extraction process with the Tissue RNA Miniprep Kit generally takes about 90 minutes to two hours from start to finish. This duration allows for cell lysis, RNA binding, washing, and elution processes to be completed efficiently. This timeframe is beneficial for labs handling multiple samples, as it enables a rapid workflow without compromising the quality of the extracted RNA, ideal for high-throughput research settings. -
Pregunta:
What storage conditions are recommended for the kit components?
Respuesta: The kit components, including the buffers and DNase I, should be stored at 4°C for short-term use. If not used, it is recommended to store the kit at -20°C for long-term stability to prevent degradation or loss of activity. Following these storage guidelines helps ensure that the kit components maintain their integrity and functionality, providing reliable results across multiple uses. -
Pregunta:
Where can I buy the Tissue RNA Miniprep Kit with DNase I, 50 preps?
Respuesta: You can buy the Tissue RNA Miniprep Kit with DNase I, 50 preps on Ubuy, which offers a reliable platform for a variety of laboratory and research products. Ubuy ensures that customers have easy access to high-quality kits essential for RNA extraction and analysis, making it a valuable resource for researchers looking for effective solutions in molecular biology.
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Customer Reviews & Ratings
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ventajas
- High yield of RNA
- Effective DNase treatment
- User-friendly instructions
- Fast processing time
- consistent results
Contras
- Buffer solutions have a short shelf life.
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CRC 167119
Haz tu pedido ahora y recíbelo por ahí Viernes, Octubre 02
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características y beneficios
- Easy and fast RNA isolation in 30 minutes
- Purifies up to 100 µg of total RNA from tissues and cultured cells
- RNA purification suitable for RT-PCR, Northern blotting, polyA+ RNA purification, nuclease protection, and in vitro translation
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