What is a Phosphate Buffer, pH 7.2 used for?

A Phosphate Buffer, pH 7.2 is commonly used in biological and chemical laboratories to maintain a stable pH environment essential for various experiments. This buffer solution is particularly beneficial in enzymatic reactions, cell culture, and molecular biology applications where pH fluctuation could impact the outcome. For instance, many enzymes exhibit optimal activity at specific pH levels, and using this buffer can help maintain those conditions.

How do you prepare a Phosphate Buffer, pH 7.2?

To prepare a Phosphate Buffer, pH 7.2, a precise mixture of disodium hydrogen phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) is required. This involves dissolving a calculated amount of each salt in distilled water, and adjusting the pH to 7.2 using a pH meter. This method is crucial for applications requiring accurate pH values, such as biochemical assays or cell culture media preparations.

What are the advantages of using a Phosphate Buffer?

Phosphate Buffers are widely favored due to their ability to maintain stable pH levels with minimal changes upon dilution or during ionic strength variations. They are effective in a physiological range, making them ideal for biochemical reactions that mimic in vivo conditions, such as protein purification and cellular experiments. Additionally, they exhibit low absorbance at UV wavelengths, making them suitable for spectrophotometric analysis.

Can I use Phosphate Buffer for cell culture?

Yes, Phosphate Buffer, pH 7.2 is frequently used in cell culture applications. It helps maintain the physiological pH that is crucial for cell viability and function. In cell culture media, this buffer plays a vital role in providing the stability needed for optimal cell growth and productivity, allowing researchers to conduct experiments without adverse effects from pH fluctuations.

Is Phosphate Buffer suitable for protein assays?

Absolutely, Phosphate Buffer, pH 7.2 is highly suitable for protein assays. Many analytical methods, such as enzyme-linked immunosorbent assays (ELISA) or spectrophotometric analyses, rely on a stable pH environment to ensure accurate results. The buffer's ability to maintain consistent pH levels helps ensure that the proteins retain their biological activity and structural integrity during analysis.

What should I consider when storing Phosphate Buffer?

When storing Phosphate Buffer, pH 7.2, it's important to keep it in a tightly sealed container away from direct light and temperature extremes. Exposure to air may lead to contamination or evaporation, altering its pH. Proper storage ensures that the buffer remains effective for laboratory use, especially for sensitive experiments requiring pH control.

Can Phosphate Buffer be used in chemical synthesis?

Yes, Phosphate Buffer, pH 7.2 can be utilized in various chemical synthesis processes. It can help in maintaining the desired pH during reactions involving acid-base chemistry, where a stable pH is crucial for the desired outcomes. This buffer is often employed in reactions such as esterification or polymerization, where pH stability influences the reaction rate and mechanism.

Is Phosphate Buffer compatible with all biochemical reactions?

While Phosphate Buffer is versatile, it's not suitable for all biochemical reactions. Some reactions may be sensitive to phosphate ions or require a different pH range. For instance, reactions involving magnesium-dependent enzymes may necessitate using alternative buffering systems. Therefore, it's essential to evaluate the specific requirements of your experiment to ensure compatibility.

What is the concentration of Phosphate Buffer, pH 7.2?

The Phosphate Buffer, pH 7.2 is available in a concentration of 1.0 M, which indicates that it contains 1 mole of phosphate ions per liter. This concentration is often preferred for laboratory applications because it provides effective buffering capacity and maintains stability against pH changes during biochemical processes. Higher concentrations might be too strong for some applications, while lower concentrations could compromise the buffering ability.

Where can I buy Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml?

You can easily purchase Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml from Ubuy in Costa Rica. Ubuy offers a wide selection of laboratory reagents, including this buffer solution, ensuring a convenient shopping experience for your laboratory needs.

¿Cómo comprar Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml en línea desde Ubuy?

Es fácil comprar Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.

¿Está Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml disponible para comprar en línea en Costa Rica?

Sí, en Ubuy Costa Rica, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.

¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

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Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers offer unparalleled quality for your molecular biology research.

eCon Lab Supply

Nº de artículo: 175546200

Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml

Nº de artículo: 175546200

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What Stands Out

Optimal pH Stability

This phosphate buffer maintains a stable pH of 7.2, ensuring reliable results in various biological and chemical experiments, reducing the risk of fluctuations that could compromise study integrity.

Concentrated Formula

With a concentration of 1.0 M, this buffer allows for easy preparation of dilutions, facilitating precise and controlled experimental conditions crucial for research and laboratory work.

Versatile Applications

Ideal for molecular biology and biochemical assays, this buffer is compatible with a wide range of applications, ensuring flexibility and reliability for lab professionals across various research fields.

Detalles de producto

Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.88
Item Weight	8 ounces (226.8 grams)
Brand	eCon Lab Supply

Who Should Buy?

Biologists
Ideal for experiments requiring stable pH conditions, ensuring optimal enzyme activity and accurate molecular biology workflows.
Chemists
Useful for maintaining pH in various chemical reactions, enhancing reliability in laboratory experiments and formulations.
Healthcare labs
Essential for diagnostic procedures or assays that require precise pH levels, improving test accuracy and patient outcomes.

Educational use
May not suit beginners as handling buffers requires some chemistry knowledge and experience for safety and effectiveness.

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Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml

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Preguntas y respuestas de los clientes

Pregunta: ¿Cómo comprar Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml en línea desde Ubuy?

Respuesta: Es fácil comprar Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml en línea desde Ubuy.. Solo tiene que buscar el producto, elegir su método de envío al pagar y recibirlo en su ubicación.
Pregunta: ¿Está Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml disponible para comprar en línea en Costa Rica?

Respuesta: Sí, en Ubuy Costa Rica, este producto está disponible para que lo compre a un precio razonable.. El Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml no está disponible localmente, pero puede confiar en nosotros con nuestros servicios de envío exprés.
Pregunta: ¿Cuánto tiempo se tarda en obtener el producto después de realizar el pedido?

Respuesta: El tiempo de entrega de su producto pedido varía según lo que haya pedido y el método de envío que haya elegido.. El tiempo de entrega estimado se menciona durante el proceso de pago, así que no se preocupe mientras compra.

eCon Lab Supply Buffers Editorial Review

Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml is a reliable solution from eCon Lab Supply, designed for laboratory applications requiring precise pH control. This buffer is packaged in a sturdy bottle, ensuring easy handling and storage. Users appreciate the consistent performance and effectiveness in maintaining stable pH levels during experiments, which is critical for various biochemical procedures. The 250 ml volume is suitable for routine use, allowing for adequate supply without waste. The specifications indicate that it is manufactured by Ciro Mfg, which is known for quality lab supplies.

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ventajas

High-quality phosphate buffer for lab use
Stable pH maintenance for experiments
Convenient 250 ml bottle size
Lightweight design at 8 ounces
Trusted brand with reliable performance

Contras

Not suitable for very high pH adjustments

Product Price History

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Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.

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características y beneficios

Comprehensive range of biological buffers for research and diagnostics.
Ideal for protein research, molecular biology, and characterization.
Manufactured in USA in ISO 9001 and 13485 certified facility.
Produced under stringent Good Manufacturing Practices.
Quality control tests conducted in certified 14644 clean room conditions.
Perfect for immunochemical staining and preserving living tissues.

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Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml

CRC 34006

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100% Original Products

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What Stands Out

Detalles de producto

Who Should Buy?

DESCRIPCIÓN DEL PRODUCTO

Preguntas y respuestas de los clientes

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

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ventajas

Contras

Product Price History

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CRC 34006

características y beneficios

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